Part:BBa_K3885352

P28-tetO-deGFP-ssrA

Under the P28 promoter, the Tet operator tetO is expressed along with the production of green fluorescent protein.Under the degradation of ssrA, the tet operator tetO is progressively degraded.

Usage and Biology

1. This plasmid has ssrA tag. Compare with P28-tetO-deGFP, we use it to investigate the effect of degradation ssrA tag on deGFP fluorescent protein.
2. It can be used in other experiments which hope to express fluorescence with ssrA.

Characterization

This part provides ssrA tag. Compared with P28-tetO-deGFP, we can prove that degradation label will decrease the expression of fluorescent protein.
The result shows that the element which has no ssrA can present more fluorescence intensity.

Design Page

We want to know if the network would exhibit a pulse-like signal in TXTL when protein degradation machinery was added.

Usage and Biology

Melissa K. Takahashi et al. Characterizing and prototyping genetic networks with cell-free transcription–translation reactions[J]. Methods, 2015, 86 : 60-72.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]